Note that this workshop is a high-level introduction to the analysis of large datasets from a user perspective. We will exploit a number of Bioconductor packages able to interact with the HDF5 on-disk data representation, freeing us of the need to load the full dataset in memory. The proposed workflow consists of the following steps: We focus on the most common application in exploratory single-cell analysis, namely to find subpopulations of cells. This workshop illustrates the latest Bioconductor infrastructure to analyze large single-cell datasets that may not entirely fit in memory.
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